Virus-mediated expression of Phytophthora sojae avirulence (Avr) proteins

July 30, 2018

Fides Zaulda, Ph.D. Student, Department of Plant Pathology The Ohio State University

Advised by Dr. Feng Qu

Virus-mediated expression of Phytophthora sojae avirulence (Avr) proteins

We use Bean pod mottle virus (BPMV) to express P. sojae Avr proteins to elicit hypersensitive response and screen for soybean lines containing the corresponding Rps gene

Development of a Cowpea severe mosaic virus (CPSMV) infectious clone

We generated a CPSMV infectious clone that can infect soybean and tobacco. We will next attempt to develop it into a viral vector for virus-induced gene silencing and foreign protein expression in soybean

Genome editing of soybean using CRISPR

We are using CRISPR/Cas9 to edit single nucleotides in soybean in order to engineer novel herbicide resistance traits